Article in Japanese

Utility of Quenching Probe PCR for a diagnosis of macrolide resistance Mycoplasma pneumoniae in children

Ryosuke SUZUI^1,2), Yoshihiko KAWANO^1,3), Shotaro ANDO¹⁾, Jun-ichi KAWADA²⁾, Yoshinori ITO²⁾

Mycoplasma pneumoniae (M. pneumoniae) infection is a major causative organism of lower respiratory tract infection in children, and recently, macrolide tolerance has become a problem. This study retrospectively investigated utility of the Quenching Probe PCR(QP-PCR) from collected data from 123 hospitalized pediatric patients with M. pneumoniae. Clinical and laboratory data including macrolide sensitivity, age, sex, use of antibacterial drugs, white blood cell counts, lactate dehydrogenase levels, C-reactive protein levels, use of steroids, asthma symptoms, use of oxygen, length of hospital stay, and fever period were collected. Of the 123 patients, 71 were in the macrolide-sensitive group (S-group) and 52 the macrolide-resistant group (R-group) excluding one case in which macrolide tolerance were altered from S-group to R-group during the same hospital stay. In comparing between the S- and R-group, no difference in the fever period after alteration to antibacterial drugs was observed(p=0.73). The duration of total fever (p<0.01). and hospitalization (p=0.02) was significantly shorter in the group in which antibacterial drugs were consistent with results of the QP-PCR method. In diagnosing pediatric M. pneumoniae infection, its fever duration can be shortened by selecting appropriate antibacterial drugs according to the results of QP-PCR.

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1) Department of Pediatrics, Okazaki City Hospital
2) Department of Pediatrics, Nagoya University Graduate School of Medicine
3) Department of Pediatrics, TOYOTA Memorial Hospital

Key words	Mycoplasma pneumoniae, Macrolide resistant Mycoplasma pneumonia, PCR, Quenching Probe
Received	February 18, 2019
Accepted	June 4, 2019

31 (3)：247─254,2019